Adhesion of Sphingomonas sp. GY2B onto montmorillonite: A combination study by thermodynamics and the extended DLVO theory.

Abstract Bacterial adhesion on mineral surface are of fundamental importance in geochemical processes and biogeochemical cycling, such as mineral transformation and clay-mediated biodegradation. In this study, thermodynamics analysis combined with classical Derjaguin-Landau-Verwey-Overbeek (DLVO) theory as well as the extended DLVO (XDLVO) theory were employed to investigate the adhesion of the Gram-negative PAH-degrading bacteria Sphingomonas sp. GY2B on montmorillonite (Mt). Scanning electron microscopy (SEM), Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS) indicated the affinity of GY2B for Mt, and the experimental results could be described well by pseudo-second-order (R2 = 0.997) and Langmuir model (R2 = 0.995). The thermodynamics analysis revealed the physical nature of bacterial adhesion onto Mt, which was confirmed by the XDLVO theory. The related surface properties (Zeta potential, hydrodynamic diameter and hydrophobicity) at different ionic strength were determined and the interaction energy between Mt and GY2B were also calculated using the DLVO and XDLVO theories in KCl or CaCl2 solution. At low ionic strength (≤ 20 mM), GY2B adhesion onto Mt was primarily driven by long-range DLVO forces (e.g. electrostatic repulsion), while short-range (separation distance