Distinct age-related changes in the urinary catabolic profile of prostaglandin F2 alpha in the rat.

Abstract Seven urinary catabolites were identified after intravenous infusion of tritium-labeled prostaglandin F2 alpha in the adult rat. Most products were the result of oxidation at C-15, reduction of the delta 13,14 double bond, and double beta oxidation together with omega oxidation reactions yielding 14 and 16 carbon compounds. While the former sequence of reactions is also demonstrable in the neonate (2 weeks and older), the omega oxidation system develops around 28 to 32 days of age. Consequently, the urinary catabolic pattern resulting from infusion of tritiated prostaglandin F2, in the rat younger than 32 days is composed principally of one product identified as 15-keto-13,14-dihydrotetranor prostaglandin F2 alpha (VII). This product is one of the transient intermediates in the adult and is catabolized further by omega oxidation.