Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects

Two novel actinobacteria, strains NEAU-CX1T and NEAU-JC6T, were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1T showed it to be most closely related to Micromonospora coxensis JCM 13248T (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821T (99.1 %) and Micromonospora halophytica JCM 3125T (98.6 %), and that of strain NEAU-JC6T to Micromonospora haikouensis DSM 45626T (99.3 %), Micromonospora carbonacea JCM 3139T (99.1 %) and Micromonospora krabiensis JCM 12869T (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1T showed it to be most closely related to M. purpureochromogenes DSM 43821T (98.0 % sequence similarity), and that of strain NEAU-JC6T to M. haikouensis DSM 45626T (99.0 %) and M. carbonacea JCM 3139T (98.0 %). A combination of DNA–DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1T and NEAU-JC6T represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1T (=CGMCC 4.7175T = JCM 30056T) and NEAU-JC6T (=CGMCC 4.7173T = JCM 30055T).