PCR assay for detection of Mycoplasma hyopneumoniae and its clinical application

The present study was conducted to develop a PCR method for detecting Mycoplasma pneumoniae of swine(MPS) and its clinical applications.A pair of primer was designed for the detection of Mycoplasma hyopneumoniae(Mhp) according to the P36 protein gene sequence(Accession number: X67286) of J strain,and the PCR method for rapid detection of Mhp was developed.It was found that 948 bp of Mhp specific band can be amplified and 0.735 ng of Mhp DNA can be detected by the PCR assay,with no observation of the Mycoplasma gallisepticum,pseudorabies virus,eircovims,swine influenza virus,porcine reproductive and respiratory syndrome virus.The homology of cloned fragment was found to be 99.9% with the P36 gene of Mhp J strain(GenBank).A total of 42 Mhp suspected pathogenic samples was collected from different places of Guangxi and diagnosed by the PCR assay.Out of 42 samples,13 samples were positive for Mhp,which was accounting for 31%.Therefore,this PCR method could be applied for clinical diagnosis of the pathogen and provide basis for controlling MPS.