BIOSYNTHESIS OF BIOSURFACTANT BY PSEUDOMONAS AERUGINOSA USING CASSAVA FLOUR INDUSTRIAL WASTEWATER AS MEDIA

Biosynthesis of biosurfactant by  Pseudomonas  aeruginosa  have been prepared using cassava flour industrial wastewater ( manipueira ) as medium. The optimum condition of the biosurfactans biosynthesis was obtained using media containing nutrient broth and manipueira without centrifugation (NBM) with 4 days fermentation. UV-Vis and FT-IR spectra indicated that the biosurfactant was a rhamnolipid containing hydroxyl, ester, carbocylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 576  mg/L and surface tension value of 0.045  N/m. The biosurfactant was able to decrease the interface tension and form emulsion with benzene, toluene, gasoline and palm oil. This biosurfactant showed w/o emulsion system.