Method for preparing diglyceride through solventless continuous enzymolysis

2014 
The invention discloses a method for preparing diglyceride through solventless continuous enzymolysis, which comprises the following steps: stirring and mixing a grease material and glycerol; and continuously reacting through a glass column provided with immobilized lipase to obtain a diglyceride-containing mixed product, wherein the reaction temperature is 50-60 DEG C. According to the invention, column type continuous enzymolysis is adopted to directly obtain the diglyceride crude product, thereby being beneficial to product separation and being also beneficial to the stability and recycling of the enzyme. In the crude product obtained by the invention, the diglyceride content is 70% or so, and the fatty acid content is lower than 5%; and after molecular distillation, a diglyceride product of which the purity is greater than 85% can be obtained.
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