Changes in the activity of NADH-oxidase in rat tissues during experimental diabetes.

: The effects of alloxan diabetes and its reversal with insulin treatment, on NADH-oxidase (E.C.1.6.99.3) was measured in the microsomal fractions of brain, liver and kidney at different time interval after diabetes induction. A significant increase was found in the enzyme activity in brain and kidney microsomes of the diabetic animals, whereas liver showed a decrease. The decrease in the NAD+/NADH ratio in the diabetes reported earlier could be due to the changes in the enzyme activity as well as other changes in the metabolite concentration.