Development and Laboratory Testing of an Automated Monitor for the Measurement of Atmospheric Particle-Bound Reactive Oxygen Species (ROS)

Previous studies have found significant quantities of oxidative species associated with airborne particulate matter. Although oxidative stress is thought to be an important part of the mechanism by which particles produce adverse health effects, the lack of a suitable method to measure ROS on a routine basis has resulted in no work being undertaken to assess the effects of particle-bound ROS on health. In order to fill this need, an automated monitor for the continuous sampling of ambient aerosol and the measurement of concentrations of ROS on the sampled aerosol was developed. Potential methods to quantify ROS were compared in order to arrive at a suitable method to automate. The dichlorofluorescein (DCFH) fluorescence method was found to be the most non-specific, general indicator of particle-bound oxidants. Hence it was deemed the best suited method for the automated monitor. An integrated sampling-analysis system was designed and constructed based on collection of atmospheric particles in an aqueous s...