Structural assessment of theN-linked oligosaccharides of cell-CAM 105 by lectin-agarose affinity chromatography

TheN-linked oligosaccharides of cell-CAM 105, a glycoprotein involved in the intercellular adhesion between rat hepatocytes, were studied by sequential lectin-agarose affinity chromatography of desialylated, [14C]-labelled glycopeptides. These glycopeptides were obtained by extensive pronase digestion followed byN-[14C]acetylation of the peptide moieties and desialylation by mild acid hydrolysis.