PTA wastewater molecular toxicity detected with gene chip

The purified terephthalic acid (PTA) petrochemical wastewater molecula r toxicity detected by use of Mouse Genome 430A 2.0 GeneChip was conducted in th is research. The toxic dose to male mice was 0.03 g/(kg·d) of PTA in the wa stewater. The mice liver total RNA was isolated as the temple for synthesis of cDNA and then the cDNA as the temple for synthesis of cRNA. Hybridizing the cRN A with the target genes on the gene chip, there were 232 genes expression levels up-regulated and 74 genes down-regulated discovered obviously. The foremost 40 genes for both the highest and the lowest expression levels involved endogenetic steroid and hormone metabolism, immune system, the leukocyte activity and infla mmation, detoxification in liver, reproduction and growth hormone, regulation im mune factors of anti-tumor and anti-infection and cancer to the mice sampled. Th e data suggest the PTA wastewater contained over 5 aromatics and their toxicitie s integrated were much higher than the pure chemical PTA. And the pure chemical PTA toxicities data cannot be used to evaluate the toxicity of the PTA wastewate r instead.