Subcellular Localization of Avian Influenza Virus NS1 Protein

In order to deeply study on biological function of NS1 protein,learn about distribution and subcellular localization of NS1 protein in mammalian cells.NS1 gene of avian influenza virus was amplified by RT-PCR,and cloned into vector pEGFP-N1.Recombinant plasmid was identified by enzyme digestion and sequencing,the recombinant plasmid pEGFP-N1-NS1 was transfected into 293T cells and Hela cells by liposome,distribution and subcellular localization of NS1 protein was observed by the fluorescence microscope and the confocal laser scanning microscope.Eukaryotic recombinant expression plasmid pEGFP-N1-NS1 was successfully constructed,it had high levels of expression.in 293T cells and Hela cells.NS1 green fluorescent fusion protein is mainly distributed in the nucleus,is slightly distributed in the cytoplasm.The study successfully expressed NS1 protein in mammalian cells and analyzed subcellular localization of NS1protein.It is a solid basis for related function research on influenza virus.