Antibacterial activity on some gram positive and gram negative bacteria and antihelmintic activity on Tubifex tubifex worm of methanol extract of Macaranga denticulata (MUELL. ARG.) bark

The aim of the present study was to evaluate antibacterial activity on three Gram positive and four Gram negative bacteria and antihelmintic activity on Tubifex tubifex worm of methanol extract of M. denticulata (Muell. Arg.) bark. The extract showed zone of inhibition in highest concentration of 900 μg/ml against Gram-positive bacteria Staphylococcus aureus (8mm), Bacillus subtilis (12mm), Bacillus cereus(8mm)and Gram-negative bacteria Salmonella typhi (12mm), Salmonella paratyphi (Nil), Escherichia coli (13mm), Pseudomonas aeruginosa (11mm).M. denticulata bark extract exhibited strong anthelmintic activity in vitro. Where it paralyzed (6.52±0.53 min) and produced death (12.36±0.81 min) of the Tubifex tubifex at 20 mg/ml dose near the value of the standard, Levamisole (3.3±0.38 min and 6.5±0.76 min) at 1 mg/ml. So methanol extract of M. denticulata barks showed moderate antibacterial activity and well antihelmintic activity.