Genetic diversity of some tobacco germplasms by using SSR technique.

32 tobacco varieties were analyzed by SSR technique to study their genetic diversity.The DNA of 32 tobacco varieties was amplified by 8 pairs of polymorphism primers selected from 38 pairs of SSR primers.There were 70 polymorphism bands in 93 DNA amplification bands and the average polymorphism rate was up to 75.27%.32 tobacco varieties were divided into 4 kinds and the genetic distance was from 0.31551 to 1.51978 after the cluster analysis.The result of cluster analysis basically accords with the theoretical expected value,which indicates that the SSR technique can reveal the genetic background of tobacco germplasm resources and their blood relationship.