Use of aminopeptidase substrate specificities to identify species of Cylindrocladium in Wisconsin nurseries

Evaluation of an aminopeptidase assay indicated that this technique can be used to distinguish species of Cylindrocladium. Percent hydrolysis of 19 aminopeptidase substrates was determined for 22 isolates of known species identity. Discriminant analysis was used to develop models for identifying isolates of unknown species of Cylindrocladium from diseased conifer seedlings in Wisconsin nurseries. Both C. scoparium and C. floridanum were present in two nurseries.