PL-008 Systemic leishmaniasis are inhibited by acetylsalicylic acid via nitric oxide pathway in Leishmania major infected susceptible Balb/c mice

Objectives: Leishmaniasis is a zoonotic disease caused by Leishmania parasites ranging from lesion to severe cutaneous/visceral leishmaniasis. Nitric oxide as a free radical released during an inflammatory response and involved in the microbicidal activity of macrophages. Acetyl salysilic acid inhibits inflammatory reactions but little is known about its effects on CL therapy. The purpose of this study was to evaluate antileishmanial effects of ASA via NO pathway in susceptible Balb/c mice infected with L. major. Methods: Experimental leishmaniasis was initiated by injection of Leishmania promastigotes into mice. ASA was inoculated orally after lesion appearance using gavages once a day up to 13 weeks. The development of lesion was determined weekly and animals were humanely killed and target tissues were removed, weighted and their impression smears prepared. Griess microassay was applied for measurement of NO in plasma and target organs. Results: Results showed ASA increased NO production in plasma of both naive and leishmania groups. A sharp decline was observed in proliferation of amastigotes inside MQ. Contrary, ASA reduced lesion size inhibited leishmania visceralisation in spleen lymph node and decreased routine hepato/splenomegaly. However it had some negative side effect on survival rate and body weight. Results indicated some antileishmanial effects of ASA by alterations of NO as immunomodulatory factor in L. major infected Balb/c mice. Conclusion: ASA presented its ability to elevate NO concentration in plasma during systemic leishmaniasis in mice and it decreased parasite visceralization in target organs as well as declining its proliferation inside macrophages with less effect on lesion size. It presented no significant effects on hepato/splenomegaly and decrease survival rate and body weight. It is indicated ASA may be applied for inhibition of systemic leishmaniasis via nitric oxide pathway on Balb c mice infected with L. major, however more studies are required to clarify this concept. OL-036 Prevalence of canine scabies in Korean stray dogs S.-S. Yoon1 *, J.-W. Byun1, S.-H. Wee1, J.-Y. Song1. 1National Veterinary Research & Quarantine Service, Republic of Korea