Multiphoton Excitation Microscopy for Identification and Operational Control of Extracellular Matrix Components of Body Tissues

The process of extraction of the extracellular matrix (ECM) requires operational monitoring, since there is a risk of destruction of the main proteins of the cell matrix at non-optimal parameters of the process. Using multiphoton microscopy the structure of collagen, the integrity of the cell membrane and the content of elastin in the process of isolation of ECM from various tissues of the rat body (brain, dermis, and muscle) has been studied. It has been shown that multiphoton microscopy is an effective tool for monitoring the quality of ECM extraction.