Improving resistance of Japonica varieties Shengdao13 and Shengdao14 to rice stripe virus disease by molecular marker-assisted selection.

【Objective】 To develop new varieties and germplasms resistance to rice stripe disease (RSD),the molecular marker-assisted selection (MAS) strategy was used to improve the resistance of susceptible varieties Shengdao 13 and Shengdao14. 【Method】 Molecular markers closely linked to Stv-bi were used for MAS. Shengdao 13 and Shengdao14 were used as receptor parents,resistant variety Zhendao 88 and the high generation of disease-resistant rice strain Shengdao519 were used as the donor parents,applied self-improvement and backcross breeding methods,using MAS,combined with field resistance to RSD identification and agronomic traits analysis,Stv-bi (or allele gene) was successfully transferred to Shengdao13 and Shengdao14. 【Result】 A number of strains carrying the disease-resistance gene had been obtained through two inbred compound breeding populations,and six backcross stable lines carrying Stv-bi gene E01,E13,R107,etc. were obtained. 【Conclusion】 MAS can significantly improve the selection efficiency and accuracy in resistance breeding,MAS breeding system for stripe disease was established. The results have provided theories and materials for the future resistance breeding.