PHOTOCHEMICAL SCREENING OF GITAAN ROD ETHANOL EXTRACT (Willughbeia firma Blume) USING THIN LAYER CHROMATOGRAPHY METHOD

Gitaan (Willughbeia firma Blume) is one of the forest plants in South Kalimantan, the stems of which have been trusted for generations by the people of the Halong area, Balangan district, as a medicinal plant that can treat food poisoning containing heavy metals. The benefits of the stems of the Gitaan plant are because the stems of Gitaan contain secondary metabolite compounds. No research has been found on secondary metabolites of Gitaan stem extract. To identify the content of secondary metabolites in the ethanol extract of Gitaan stems (Willughbeia firma Blume) using Thin Layer Chromatography (TLC). This study used a descriptive observational method obtained from the secondary metabolite content of the Gitaan stem ethanol extract by observing the color reaction of the spots on TLC (qualitative data) and Rf value. The results of the color test of the Gitaan stem ethanol extract were positive for secondary metabolites of alkaloids, flavonoids, terpenoids, tannins, and saponins. The best eluent for separating flavonoid compounds is ethyl acetate: chloroform (12:6), dichloromethane alkaloids: ethyl acetate (10:10), hexane terpenoid compounds: ethyl acetate (12:8), methanol tannin compounds: ethyl acetate (8 :2), and the saponin compounds are chloroform: methanol: water (15:5:1). The results of the positive spray reaction showed secondary metabolites of alkaloids, flavonoids, terpenoids, tannins and saponins. The secondary metabolite compounds in the Gitaan stem ethanol extract that have the most potential to overcome poisoning are tannins and flavonoids. The best eluent for separating flavonoid compounds is ethyl acetate: chloroform (12:6), dichloromethane alkaloids: ethyl acetate (10:10), hexane terpenoid compounds: ethyl acetate (12:8), methanol tannin compounds: ethyl acetate (8 :2), and the saponin compounds are chloroform: methanol: water (15:5:1). The results of the positive spray reaction showed secondary metabolites of alkaloids, flavonoids, terpenoids, tannins and saponins. The secondary metabolite compounds in the Gitaan stem ethanol extract that have the most potential to overcome poisoning are tannins and flavonoids. The best eluent for separating flavonoid compounds is ethyl acetate: chloroform (12:6), dichloromethane alkaloids: ethyl acetate (10:10), hexane terpenoid compounds: ethyl acetate (12:8), methanol tannin compounds: ethyl acetate (8 :2), and the saponin compounds are chloroform: methanol: water (15:5:1). The results of the positive spray reaction showed secondary metabolites of alkaloids, flavonoids, terpenoids, tannins and saponins. The secondary metabolite compounds in the Gitaan stem ethanol extract that have the most potential to overcome poisoning are tannins and flavonoids. The results of the positive spray reaction showed secondary metabolites of alkaloids, flavonoids, terpenoids, tannins and saponins. The secondary metabolite compounds in the Gitaan stem ethanol extract that have the most potential to overcome poisoning are tannins and flavonoids. The results of the positive spray reaction showed secondary metabolites of alkaloids, flavonoids, terpenoids, tannins and saponins. The secondary metabolite compounds in the Gitaan stem ethanol extract that have the most potential to overcome poisoning are tannins and flavonoids.