Derivative of naturally occurring g-csf, its preparation and dna base sequence encoding amino acid sequence of derivative of naturally occurring g-csf

PURPOSE: To prepare a colony stimulating factory having stability. CONSTITUTION: This deriv. of naturally occurring G-CSF has at least one of biological activities of naturally occurring G-CSF and at least 35% soln. stability at 5 mg/ml and has at least Cys 17 of the native sequence replaced by an Ser 17 residue and Asp 27 of the native sequence replaced by an Ser 27 residue. Suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells can be transformed or transfected by incorporating nucleotide sequences encoding part or all of the amino acid sequence of the G-CSF deriv. into autonomously replicating plasmid or viral vectors. COPYRIGHT: (C)1994,JPO