Сравнительный анализ методов экстракции общей геномной ДНК голубики высокорослой

Two protocols of DNA extraction from Vaccinium corymbosum leaves and stems (according to Li et al., 2007, with modifications; according to Dempster et al., 1999, with modifications) were tested. Both protocols were designed for application to the tissues with high contents of PCR inhibitors and based on thermal lysis in buffer containing CTAB, EDTA, NaCl, 2-mercaptoethanol and PVP. The first protocol includes extraction by phenol, chloroform and isoamyl alcohol, and DNA precipitation by ethanol. The second includes extraction by chloroform and isoamyl alcohol, and DNA precipitation by isopropanol. DNA extracts obtained by the second protocol were of high purity and provided enough products in ISSR-PCR.