Arl13b interferes with α-tubulin acetylation

Methods We used the zebrafish Kupffer’s vesicle as a dynamic ciliary growth system and performed a seven hour timecourse experiment comparing the length of cilia measured by Arl13b-GFP or by acetylated a-tubulin. In order to evaluate the specificity of the alterations in a-tubulin acetylation pattern, we overexpressed different ciliary proteins that were also reported to increase cilia length.