Hydrogenophaga aquatica sp. nov., isolated from a hot spring
2017
A polyphasic approach was used to characterize an aerobic, Gram-negative, rod-shaped bacterium (designated strain CC-KL-3T) isolated from a hot spring. Phylogenetic analyses based on 16S rRNA genes indicated that strain CC-KL-3T showed highest sequence similarity to
Hydrogenophaga bisanensis
(97.7 %) and
Hydrogenophaga atypica
(97.6 %) and lower sequence similarity to other species (less than 97.6 %). The levels of DNA–DNA relatedness between strain CC-KL-3T,
H. bisanensis
and
H. atypica
were estimated to be 13.0 and 8.7 % (the reciprocal value was 14.7 and 6.3 %). Strain CC-KL-3T was non-motile, without apparent flagella and able to grow between 15–42 °C (optimal 30 °С), pH 6.0–8.0 (optimal 7.0) and 0–2 % (w/v) NaCl (optimal 0 %). The DNA G+C content was 61.4 mol% and the major quinone system was ubiquinone (Q-8). The polyamine profile revealed the predominance of 2-hydroxyputrescine and putrescine and the dominant cellular fatty acids were C16 : 0 (28.9 %), C16 : 1ω7c/C16
: 1ω6c (41.4 %) and C18 : 1ω7c/C18
: 1ω6c (11.9 %). These data corroborated the affiliation of strain CC-KL-3T to the genus
Hydrogenophaga
. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits, and the results of comparative 16S rRNA gene sequence analysis, strain CC-KL-3T is considered to represent a novel species of the genus
Hydrogenophaga
, affiliated to the family
Comamonadaceae
, for which the name Hydrogenophaga aquatica sp. nov. is proposed. The type strain is CC-KL-3T (=BCRC 80937T=JCM 31216T).
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