Molecular Cloning Segment S11 and S12 Of RDV SynChrOnOuSIy by Single Primer Amplification and Sequence Analysis

The 10%SDS-PAGE of dsRNA profiles from six RDV isolates showed:the relative velocity of Gegment S11,S12from RDV-SX isolate was obviously dissimilar from others.The full length cDNAS of RDV—SX isolate genome segments S11.S12were cloned by Single Primer Amplification and their sequences were determined.The result showed the full length of S11,S12 is1036bp,1066hp respectively and has the same organization with RDV in Fuzhou.The similarity of nucleotide was 97.01%,97.86%with Fuzhou isolate respectively.This study provided a good method for the sequence determination of viral dsRNA atthe sametime.