Relationship between cytokine gene polymorphism and development of gastric adenocarcinoma

Objective To investigate the relationship between the single nucleotide polymorphisms (SNP)of tumor necrosis factor-α(TNF-α)promoter genes and susceptibihty to gastric adenocarcinoma or gastric adenocarcinoma with helicobacter pylori(Hp)infection.Methods The SNPs of the TNF-α(-238G/A and-308G/A)were determined by gene chip in 130 patients with gastric adenocarcinoma and 142 healthy controls.The sera concentrations of IgG,IgM and IgA of Hp antibodies were measured by ELISA in all cases and controls.Results H.pylori infection was detected in 69.2% of the 130 patients and 46.5% of the 142 controls(P0.01).The frequencies of TNF-α-238GA genotype and A allele in the patients with gastric adenocarcinoma were significantly higher than that in the healthy controls(P0.01,P0.01).The frequencies of TNF-α-238GA genotype and A allele in the patients with gastric adenocarcinoma with Hp infection were significantly higher than that in the Hp-negtive patients with gastric adenocarcinoma(P 0.05,P0.01).No association was found between any of the other polymorphisms and the patients with gastric adenocarcinoma or patients with gastric adenocarcinoma with Hp infection.Conclusion TNF-α- 238GA genotype and A allele are significantly related to susceptibility to gastric adenocarcinoma or susceptibility to gastric adenocarcinoma with Hp infection.