Alternativas para a obtenção de uma linhagem celular contínua de abelhas Apis mellifera
2021
In worldwide there are reports of a significant decrease in colonies of the species Apis mellifera, caused by several factors,
including viral infections. In order to study and diagnose illnesses caused by viruses, in vitro cell culture is used as a valuable tool. Yet, there
are still no immortalized cell lines of honey bee Apis mellifera. Primary cell cultures are promising for this purpose and can supply the lack of
continuous strains, but their establishment is difficult and laborious, which often makes them unfeasible for many research centers. Through
the use of cell immortalization techniques, it is possible to develop continuous cell lines and thus benefit, in different ways, research related to
different species of bees. The choice of technique is challenging, since in addition to the ability to remain viable for countless passages, cells
must keep the genotype and phenotype similar or identical to the original tissue. This review intends to present methodologies that can be used
to immortalize Apis mellifera cells, aiming to establish a cell line. The genotypic and phenotypic implications of each technique are evaluated,
and the purpose of the cell line to be developed.
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