A novel translocation (x;4)(p22.1;q32) in a child with malignant Schwannoma

8572 Background: Malignant Schwannoma is a rare entity that represents 2% of the peripheral nerve sheath tumors. It usually arises from limbs, paravertebral areas or head and neck.It has an aggressive biological behavior with local infiltration and hematogeneous metastases. Multiple cytogenetics aberrations and structural anomalies have been demonstrated by some authors. A novel unreported translocation t(X;4)(p22.1;q32) in a 6 years old boy with malignant paravertebral Schwannoma, without prior exposure to radiotherapy or chemotherapy was diagnosed and demonstrated. Methods: Fresh tumor was minced and digested with Trypsin-EDTA 1:250 for 15 minutes at 37oC. The suspended material was seeded into culture flasks with RPMI medium, 20% fetal calf serum and antibiotics and incubated in 5% CO2 at 37oC. When mitotic activity was identified the cells were harvested and also the tumor within 11 days. Cells were treated with Colquicine 16uL/mL for 3 hours. A hypotonic solution of KCl 0,075M was used for 15 minutes...