Screening of LMW-GS Alleles Locating at Glu-B3 Locus in Common Wheat Micro Core Collectio

Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of common wheat. The 285 common wheat micro core collection was tested by 10 Glu-B3 site specificity maekers for the 9 alleles types on the low molecular weight glutenin Glu-B3 site. Glu-B3 allele types of 284 varieties had been identified, and 1 variety has an unknown allele. Of these, the number of c allele is the most, and reaches up to 56.49%, e and i alleles are the second which contain 32.28%. The a, b, d, f, g, alleles take up 18.60%, 27.37%, 28.77%, 12.98%, 15.79%, respectively. However, the h allele is the least of 3.55%, and the unknow allele is only 0.36%. According to the detection rate the order of nine alleles is c>i=e>d>b>a>g>f>h The c allele is more than twice of b allele which has larger effect in the gluten strength and the c allele is the smallest effect in the gluten strength. With b allele varieties can be used as quality breeding new candidate parents. Keywords Wheat (Triticum aestivum L.); Low-molecular-weight glutenin subunit; Glu-B3; PCR; micro core collection