Modelling of a DNA packaging motor

During the assembly of many viruses,a powerful molecular motor packages the genome into a preassembled capsid.The Bacillus subtilis phage 29 is an excellent model system to investigate the DNA packaging mechanism because of its highly efficient in vitro DNA packaging activity and the development of a single-molecule packaging assay.Here we make use of structural and biochemical experimental data to build a physical model of DNA packaging by the 29 DNA packaging motor.Based on the model,various dynamic behaviours such as the packaging rate,pause frequency and slip frequency under different ATP concentrations,ADP concentrations,external loads as well as capsid fillings are studied by using Monte Carlo simulation.Good agreement is obtained between the simulated and available experimental results.Moreover,we make testable predictions that should guide future experiments related to motor function.