Optimization of Brucella detection using polymerase chain reaction

: The possibility of using polymerase chain reaction (PCR) for the laboratory diagnosis of brucellosis was tested with a representative regional collection of 44 Brucella strains. The strains differed by the test and source of isolation, virulence, and phenotypical characteristics. Optimal parameters of PCR have been defined and the possibility of detecting the agents in pathological material from cattle demonstrated.