Determination of Fructose and Glucose Contents in Honey by Liquid Chromatography-refractive Index Detection Method and Direct Titration Method

In this study,fructose and glucose contents in honey were determined by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method.According to the results,there were great differences between determination results of reducing sugar contents by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method.Specifically,average determination results of reducing sugar contents by liquid chromatography-refractive index detection method were reduced by about 9.5% compared with alkaline copper tartrate solution-direct titration method.Subsequently,determination results of reducing sugar contents by two methods were compared and analyzed.Liquid chromatography-refractive index detection method was used to determine fructose and glucose monomers in honey,but alkaline copper tartrate solution-direct titration method was used to determine reducing sugar composition in honey,which might lead to significantly different results.Due to small sample size and limited conditions,the determination results were not necessarily representative.Bee product acquisition and processing enterprises and relevant departments should pay much attention on these issues and fully consider the current situation of grass-roots units to develop scientific,rigorous,simple,universal,convenient,low-cost and practicable standards,thus ensuring the safety and reliability of food quality.