Retraction: Molecular cloning and sequencing of cDNA encoding the phosphatidylinositol kinase from rat brain.

Molecular cloning and sequencing of cDNA encoding the phosphatidylinositol kinase from rat brain. Akio Yamakawa, Makoto Nishizawa, Kousaku T. Fujiwara, Sadaaki Kawai, Hirofumi Kawasaki, Kouichi Suzuki, and Tu- daomi Takenawa We previously reported the characterization of a cDNA clone isolated from XgtlO rat brain cDNA libraries. In this experi- ment, we made antibodies against the protein expressed in Escherichia coli and immunoprecipitated the solubilized frac- tion of rat brain. The antibodies were able to precipitate phosphatidylinositol 4-kinase activity. Moreover, we did not find any homologous clones in GenBank at that time. There- fore, we concluded that the clone encoded phosphatidylinosi- to1 4-kinase. But, subsequently, we noted that the nucleotide sequence of our clone had homology to a rat clone described by Suzuki et al. (Suzuki, H., Kawarabayasi, Y:, Kondo, J., Abe, T., Nishikawa, K., Kimura, S., Hashimoto, T., and Yamamoto, T. (1990) J. Biol. Chem. 265,8681-8685). Then, we asked for Dr. Yamamoto to determine whether our clone expressed in E. coli had increased long-chain acyl CoA syn- thetase activity or not. As the result, we found that the E. coli had high long-chain acyl CoA synthetase activity showing that our clone encodes the long-chain acyl CoA synthetase but not phosphatidylinositol ,4-kinase., Therefore, we would like to retract the manuscript.