The Comparation Between Topical Aplication of the Aqueous Leaf Extract of Carica Papaya (ALEC) & Khaula Honey in Accelerating Skin Wound Healing in Mice

Topical application of papaya and honey has been hypothesized to accelerate skin wound healing. The purpose ofthis research was to evaluate the differences between topical application of the ALEC and Khaula Honey inaccelerating skin wound healing in mice. The experiment took place in Histology Laboratory, School of Medicine,Padjadjaran University, Bandung, November 2006-April 2007.The prospective experimental method was held in 10days. Subjects were male ddy mice divided into 3 groups (each consisted of 9 mices), which were control groupsolcoseryl jelly, 10% ALEC in vaseline and 1.0 g Khaula honey treated group. The comparisons in accelerating skinwound healing were investigated by using full thickness skin wound model produced on the back of the mice.Solcoseryl jelly was applied topically to wound of group 1, group 2 and group 3 mice were treated topically with 10%ALEC in vaseline and Khaula honey, respectively. The mice were sacrificed on 4th, 7th, and 10th day of post wounding for evaluating the histological changes. Data was obtained by microscopically analysis of the skin based on theepidermal regeneration, granulation tissues thickness and angiogenesis and then analyzed by using parametricindependent T-test. The level for statistical significant was set p < 0.05. The result of this experiment showed thatthere were significant difference between control group and ALEC10% in vaseline in three mentioned above.Comparison between control and Khaula honey showed differences only in epidermal regeneration andangiogenesis. Wound treated with ALEC 10% in vaseline and Khaula honey group showed significantly differencein epidermal regeneration (mean 2.19 (0.81) for ALEC 10% and 2.67 (0.67) for honey group, p value < 0.001) andgranulation tissues thickness (mean 2.99 (0.94) for ALEC 10% and 3.23 (0.99) for honey group, p value 0.038).These result documented the differences of ALEC and Khaula honey for the acceleration of wound healing process infull thickness skin wound especially in epidermal regeneration and granulation tissues thickness.[ MKB . 2010;42(2):76-81]. Key words : Wound healing, Carica papaya, Khaula honey