Establishment of an indirect ELISA for detection of antibodies against NS2 protein of Gosling plague

In this study, an indirect ELISA method for detection of Goose parvovirus (GPV) was developed by using purified recombinant NS2 protein of the virus as coating antigen. The assay had a sensitivity of 100 % for positive samples and 86.67 % for negative samples. It had no cross reaction with goose positive serum of H5, H7, H9 subtypes of avian influenza virus, and goose paramyxovirus. The coefficient variation of intro-batch was 4.2 % and the coefficient variation of inter-batches was 8.3 %. The ELISA plate could be validity for storage at 4 ℃ up to 6 months. This method was a rapidly and sensitive for the detection of GPV antibody and epidemiological survey.