Study on the molecular epidemiological characteristics of HuCV among children with diarrhea in Kunming, 2014-2015

Objective To acknowledge the molecular epidemiology of HuCV among children with acute diarrhea in Kunming, and to provide scientific basis on prevention and control. Methods 321 specimens from children with diarrhea were collected at First people's Hospital of Yunnan province and Children's hospital of Kunming city between July 2014 and June 2015.RT-PCR were used to detect calicivirus. Partial sequence of RNA dependent RNA polymerase(RdRp) of the positive samples were amplified by RT-PCR, and then the PCR production were purified, sequenced and put into phylogenetic analysis. Results Of the 321 specimens, 34 positive specimens were identified and the prevalence was 10.59%(34/321). HuCV Gastroenteritis did not present any seasonal distribution, and the prevalence rates showed no differences between gender and age groups.Typing and phylogenetic analysis revealed that NoV GⅡwas the dominant strains. Conclusions HuCV was one of the most major pathogens among children with diarrhea and GⅡ.4/Sydney2012 variant was identified as the predominant strain in Kunming. Key words: HuCV; Diarrhea; Molecular epidemiology; Sequence analysis