Effects of copper and nickel on the axenic growth and phosphastase activity of an ectomycorrhizal fungus, Amanita muscaria

Amanita muscaria was grown in Palmer and Hacskaylo medium amended with 0.15, 0.30, 0.45, 0.60, 0.75, 0.90 mM of Cu or Ni per litre as CuCl 2 or NiCl 2 . The growth, protein content and acid and alkaline phosphatase activity were estimated. Amanita muscaria was more tolerant to Cu than Ni.