Comparative Study on Characterization of Three Staphylococcal Isolatesfrom Varied Origin
2013
Two strains of Staphylococcus isolated from urine and sputum samples
were compared with a laboratory strain of Staphylococcus. Since all
three grew on the same media specific for S. aureus, they were studied
for their relatedness by morphological, biochemical and molecular
characterization. Variation in properties among the isolates pointed
towards their different identity which was confirmed through 16S rRNA
gene sequence based phylogenetic analysis. The isolate A from urine was
found to be closely related to S. haemolyticus, while isolate P from
sputum was S. aureus and isolate S from NICED culture collection was
closest to S. epidermidis. Thus the Hicrome aureus agar (Himedia M1468)
was not specific for the species S. aureus and could only screen at
genus level. The antibiotic profile of the strains was evaluated using
a spectrum of 18 antibiotics. The lecithinase activity was retained
only in freshly isolated strains and totally ceased in subsequent
subculturing.
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