공복시간이 유산소 운동후 흰쥐 심장근의 미토콘드리아 생합성에 미치는 영향

Mitochondrial biogenesis is controled through the transcriptional regulation PGC-1α, NRF-1, mtTFA and AMPK. Activation of AMPK by food deprivation is linked to the changes in the protein expressions of PGC-1 and related genes. This study was performed to determine the effect of food deprivation on mitochondrial biogenesis during aerobic exercise in left ventricular muscle. To achieve this purpose, male SD-rats (12 wk) were randomly assigned into Control (feeding, n=7), 6hF (6h fasting, n=7), 1hF+Ex (1h fasting + exercise, n=7), 3hF+Ex (3h fasting + exercise, n=7), 6hF+Ex (6h fasting + exercise, n=7). The exercise group was sacrificed at the time of 3hour after acute exercise (75∼80% VO2max). Expression of related proteins were analyzed by SDS-PAGE and western blotting. From the results of this study, expression of AMPK(76%, p=.001), PGC-1α(137%, p=.001), NRF-1(84%, p=.001), mtTFA(129%, p=.001) and COX-IV(76%, p=.001) in 6hF+Ex(6h fasting + exercise) group were significantly greater than Control (feeding) group, as well as AMPK(68%, p=.001), PGC-1α(87%, p=.001), NRF-1(48%, p=.001), mtTFA(57%, p=.001) and COX-IV(67%, p=.001) in 3hF+Ex(3h fasting + exercise) group were significantly greater than Control(feeding) group. This study demonstrates that the aerobic exercise after 6h fasting has optimal effect on mitochondrial biogenesis in heart muscle.