adenoviral E1B shuttle vectors with suppressed.

Procedure for creating an adenoviral vector, said method comprising the following steps: (i) introducing unique cloning sites adenoviral E1b region in allowing selective deletion or E1b region genes; (Ii) deleting one or more E1b region genes selected from the group consisting of 55k; 19k and 55k; 19k, 55k and pIX; and pIX; and (iii) substituting said one or more genes E1b by a heterologous gene such that said heterologous gene is under the control of the promoter E1b endogenous, and so that the heterologous gene substantially present the temporal expression pattern of or genes the E1b region suppressed.