Synthesis of oligonucleotides bearing an arylamine modification in the C8-position of 2'-deoxyguanosine

C8-Arylamine-dG adducts were converted into their corresponding 5′-O-DMTr-3′-O-phosphoramidite-C8-arylamine-dG derivatives. These compounds were used for the automated synthesis of site-specifically modified oligonucleotides. The oligonucleotides were studied for their CD properties, Tm values, and their effects on primer extension assays using human DNA-polymerase β.