Construction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood group: multimerization effect on cell agglutination and application to blood typing.

1Japanese Red Cross Central Blood Center, Hiroo, Shibuya-ku, Tokyo 150, 2Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113, 3Department of Parasitology, Institute of Medical Science, University of Tokyo, 4–6–1 Shirokanedai, Minato-ku, Tokyo 108 and 4Department of Biochemistry and Engineering, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai 980–77, Japan