Comparison of DNA extraction and quantification methods to estimate bacterial load in oral cavity

Oral health is now beyond pearly white shining teeth and has been associated with overall health and quality of life. Oral cavity harbours more than 700 bacterial species, of which, around half are cultivable Estimation of microbial load and type In the oral cavity in normal and diseased state helps to understand the dynamics of host-pathogen interaction. There are studies reporting the concentration of microorganisms in the oral cavity. However, differences In terms of DNA extraction techniques and their quantitation methods affects the interpretation of results. The objective of this study is to compare commercially available DNA extraction kits and different methods of quantitation of bacterial DNA obtained from oral cavity.