An ultrastructural and morphometric study on the effect of phosphorus-32 glass microspheres (~(32)P-GMS) on the human hepatocellular carcinoma in nude mice

Objective To study anticancer effect and ultrastructural influnce of 32 P\|GMS injected to the hepatocellular carcinoma in nude mice.Methods The ultrastructural changes of tumor was examined by electron microscope and stereology . Results In treated group, a lot of tumor cells (35%～70%) were killed.Ultrastructurally,severe nuclear damage was observed in the death cells. Besides, well differentiated and degenerative tumor cells were seen.The skin and muscle neighboring to the tumor were normal. In control group, the tumor consisted of poor differentiated tumor cells, in which there were only a few of dead cells (5%).Quantitative analysis showed that Vv of RER and mitochondria , Sv of the membrane of microvilli and canaliculi in the treatment group larger than that in the control group ( P 0.01?0.05?0.01). Conclusion The results indicated that the local administration of 32 P\|GMS produced obviously the anticancer effect.