콩 발아중의 Lipoxygenase 활성 변화

Three lipoxygenase isozymes in soybean seeds are thought to be a major contributor to lipid peroxidation and generation of free radicals which may result in seed deterioration. This study was conducted to get the basic information for changing lipoxygenase activity during seed germination in lipoxygenase-lacking soybeans. Fresh weight of soybean seedling of Jinpumkong 2 and Taekwangkong increased more rapidly than that of Jinpumkong. Hypocotyls of Jinpumkong and Jinpumkong 2 were longer and thicker than that of Taekwangkong. Type Ⅰ lipoxygenase activity (pH 9.0) in cotyledon of Jinpum-kong lacking lipoxygenase-2, 3 showed higher than that of Taekwangkong, and Type Ⅰ lipoxygenase activity of two cultivars decreased continually. On the other hand, Type Ⅱ lipoxygenase activity of Taekwangkong began to increase continually two days after germination, reached to the maximum between 4 days and 5 days, and began to decrease continually five days after germination. Type Ⅰ and Ⅱ lipoxygenase activity in hypocotyls was not detected in all soybean cultivars.