丁酸钠对人乳腺癌细胞株MCF-7细胞增殖及p27(上标 Kip1)蛋白表达影响的研究

OBJECTIVE: To investigate the effect of sodium butyrate (NaB) on proliferation of human breast cancer cell MCF-7 and expression of p27(superscript Kip1). METHODS: A human breast cancer cell line MCF-7 was treated with different concentrations of NaB. A light microscope was used to find out morphological changes in carcinoma cells. The cell cycle status were investigated by flow cytometry. The expression of p27(superscript Kip1) was determined by the immunocytochemical assay. RESULTS: NaB significantly inhibited the proliferation of MCF-7 human breast cancer cells in a time and dose-dependent manner. MCF-7 treated with NaB was arrested in G0/G1 phases, (62.2±2.2)% in 2 mmol/L NaB group, (78.1±3.8)% in 4 mmol/L NaB group, and (53.1±2.4)% in control group. Compared with the blank control group. NaB groups were significantly increased, P＜0.05. The expression of p(superscript Kip1) was markedly enhanced after being treated with NaB. CONCLUSION: NaB can inhibit the growth of breast cancer cell in vitro, which might be associated with the change of p(superscript Kip1) protein expression.