Comparative Analysis of Different Methods of Scanning Electron Microscopy and Test Preparation in Biological Tissue Studies

Different methods of scanning electron microscopy in combination with different sample preparation techniques have been compared by an example of decellularized rat diaphragm matrices. It is shown that a combination of scanning electron microscopy under natural environmental conditions, allowing one to investigate tissue samples in a state similar to native, with the conventional high-vacuum microscopy of samples obtained by drying at the critical point, provides the most complete microstructural analysis of native and decellularized tissue samples.