Immune Adsorption Chromatography for Protein Purification

Immune adsorption chromatography (IAC) for the purification of proteins in on the threshold of becoming the separation method of choice in the bioprocess industry. This technology results in extremely pure protein preparations, even with a single process step. However, several problems remain. These include: 1) low throughput, due to compression and crushing of resins with attendant flow restrictions; and 2) low monoclonal antibody (MAb) efficiency and utility, due possibly to steric hinderances, diffusional limitations, and MAb accessibility.