Molecular and Pathogenic Study of Guignardia spp. Isolates Associated to Different Hosts
2014
Fungi of Guignardia genus
are commonly isolated from different plant species and most of the time, they
are characterized as endophytes. However, some species of this genus, like G.
citricarpa and G. psidii are known as causal agents of serious
diseases that affect important crops such as Citrus Black Spot and guava fruit
rot, respectively. They are also responsible for diseases that cause foliar
spots in different fruit species and also in other crops, but cause minor
damages. Despite evidences that G.
mangiferae colonizes different plant species, there are few studies about
its genetic diversity associated with different hosts. This work has the
objective to characterize Guignardia isolates obtained from different hosts and tissues by RAPD, fAFLP and DNA
sequence of ITS1-5.8S-ITS2 region, as well as to develop pathogenicity tests
through cross inoculation in citrus and guava fruits. It was observed that
molecular markers were able to discriminate isolates of different Guignardia species. Pathogenicity tests
showed that G. citricarpa caused CBS
symptoms on citrus fruits, but it did not produce any symptoms in guava fruits. G. mangiferae isolates were able to
cause rot symptoms on guava fruits, but they have not
produced any symptoms on citrus fruits. Guignardia isolates obtained from mango leaves that have not been classified in species have not
presented any symptoms in citrus and guava fruits. Although G. mangiferae is commonly isolated
asymptomatically in different plants, this work supports the evidence that this
species has a latent pathogen behavior, at least for guava plants.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
29
References
4
Citations
NaN
KQI