硒对染T-2毒素软骨细胞表达P53、Bcl-xL和Caspase-3的影响

Objective To determine the effect of T-2 toxin and selenium on the protein expression of P53, Bcl-xL and Caspase-3 in chondrocytes, and to known the molecular targets and mechanisms of T-2 toxin induced apoptosis, the protective effects of selenium in the T-2 toxin induced apoptosis. Methods The human chondrocytes were treated with T-2 toxin (1-20 μg/L) and selenium (100 μg/L) for 5 days. The protein expression of P53, Bcl-xL and Caspase-3 were determined by Western blot analysis and their mRNA expression were determined by RT-PCR. Results An increase in P53 and a decrease in expression of the anti-apoptotic factor Bcl-xL were observed in a dose-dependent manner after exposure of 1-20 μg/L T-2 toxin, while the expression of the Bcl-xL mRNA was unchanged. Meanwhile, T-2 toxin could also up-regulate the expression of both pro-caspase-3 and caspase-3 in a dose-dependent manner. Selenium down regulated P53 and Caspase-3 expression and up regulated Bcl-xL expression induced by T-2 toxin. Conclusion These data suggest a possible underlying molecular mechanism whereby T-2 toxin could induce the apoptosis signaling pathway in human chondrocytes by the regulation of apoptosis-related proteins P53, Bcl-xL and Caspase-3. Selenium has a protective effect by partly blocking the increased expression of P53 and Caspase-3, as well as decreased expression of Bcl-xL induced by T-2 toxin.