Further purification and properties of Hageman factor cofactor

Abstract 1. 1.|Hageman factor cofactor, an intermediary in the Factor XII-dependent fibrinolysis pathway, has been purified from human plasma by adsorption onto diatomaceous earth, chromatography of the eluate on CM-cellulose, and further chromatography on DEAE-Sephadex. 2. 2.|The preparations were homogeneous on acrylamide disc electrophoresis and immunoelectrophoresis, and represented a 6000-fold purification from plasma. 3. 3.|Hageman factor cofactor could be distinguished from plasmin and urokinase by the different affinity of their esterase activities towards various esters.