Drug dynamics research of salvia miltiorrhiza in rabbit plasma and aqueous humor after intravenous injection

Objectives To assess the permeability of Danshensu at blood-ocular barrier and its characteristics of pharmacokinetics by respectively measuring the concentrations of Danshensu in blood plasma and aqueous humor of the rabbit with the high performance liquid chromatography (HPLC). Methods It was an experimental study.Seventy-two white rabbits were split into three groups:control group (6 rabbits),plasm group (6 rabbits) and aqueous humor group (60 rabbits).After 0.85% salt water (control group) or salvia miltiorrhiza (1.0 g/kg) (plasma and aqueous group) was injected into the vein of auris-edge.Samples of blood and aqueous humor were obtained for analysis.The analytical column was a BDS C18stainless steed column (5μm,4.6 mm×250 mm) ; Precolumn:YWGC18;the mobile phase consists of acetonitrile and 0.01 mol/L KH2PO4 (8:92,adjusted to p H=2.8 with phosphoric acid).The UV detector was set at 279 nm; the flow rate was 1.0 ml/min;and the column temperature was ordinary temperature. Results The plasma concentration-time curves of Salvia miltiorrhiza fitted three-compartment model.t1/2β (elimination half time) :5.661 min; Cmax (peak concentration) :727.29 mg/L; Tmax (peak time) :0 min.The aqueous humor concentration-time curves fitted two-compartment model.t1/2β: 147.663 min; Cmax: 38.62 mg/L; Tmax: 25 min. Conclusions HPLC is a sensitive,specific and accurate method that can be used in pharmacokinetics research on ocular tissue of rabbit for Danshensu,Salvia miltiorrhiza that is dissolvable in water can pass through the blood-ocular barrier after intravenous injection with a relatively stable concentration of danshensu in aqueous humor resulting from a slow rate of removal. (Chin J Ophthalmol,2013,49: 835-840) Key words: Salvia miltiorrhiza; Pharmacokinetics; Aqueous humor; Blood-aqueous